| 应用ELISA定量检测转基因玉米中Bt1蛋白的研究 |
| |
| 引用本文： | 刘光明,苏文金,陈向峰. 应用ELISA定量检测转基因玉米中Bt1蛋白的研究[J]. 食品科学, 2002, 23(8): 217-221 |
| |
| 作者姓名： | 刘光明 苏文金 陈向峰 |
| |
| 作者单位： | 厦门大学生命科学学院，厦门出入境检验检疫局，集美大学生物工程学院 |
| |
| 基金项目： | 厦门科技计划资助项目(350222001109) |
| |
| 摘 要： | 应用纯化的Bt1杀虫晶体蛋白作为标准蛋白和免疫抗原,通过抗体-抗原-酶标抗体反应,建立了酶联免疫吸附测定法(ELISA),以定量检测转基因玉米中的Bt1表达蛋白。用建立的ELISA法对4种进口玉米产品进行了测定,实验结果得到了免疫印迹分析的验证,并与进口试剂盒方法的定量分析结果相一致,因而建立的ELISA法具有操作简便、快速特异、定量准确、经济实惠的优点,特别适合于大批量检测,有着良好的应用前景。
|
| 关 键 词： | 转基因玉米 Bt1蛋白 ELISA检测 |
Quantitative Assay of Bt I Protein in Genetically Modified Maize by ELISA |
| |
| LIU Guang-Ming,SU Wen-Jin,CHEN Xiang-Feng. Quantitative Assay of Bt I Protein in Genetically Modified Maize by ELISA[J]. Food Science, 2002, 23(8): 217-221 |
| |
| Authors: | LIU Guang-Ming SU Wen-Jin CHEN Xiang-Feng |
| |
| Abstract: | Based on the antibody-antigen-enzyme antibody reaction and by using puriftied Bt1 insecticidal crystal protein asboth standard protein and immunity antigen, a quantitative enzyme-linked immunosorbent assay (ELISA) method was estab-lished to detect express Bt1 protein in genetically modified maize.4 imported maize samples were determined by ELISA, Incomparison with the western-blotting & KIT assay method, the results were almost identical. It concluded that the ELISAmethod might be useful for easy,rapid,reliable and effective assay in large samples. |
| |
| Keywords: | Genetically modified maize Bt1 protein ELISA detection |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《食品科学》浏览原始摘要信息 |
|
点击此处可从《食品科学》下载全文 |
|
