| 植物提取物中茉莉酸定性定量分析 |
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| 引用本文： | 金尉,余雯静,郑利. 植物提取物中茉莉酸定性定量分析[J]. 香料香精化妆品, 2013, 0(6): 10-13 |
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| 作者姓名： | 金尉 余雯静 郑利 |
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| 作者单位： | 苏州出入境检验检疫局,江苏苏州215104 |
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| 摘 要： | 建立了植物提取物中茉莉酸的高效液相色谱（HPLC）-三重四极杆复合线性离子阱（QTrap）质谱测定方法，样品经提取后，采用反相色谱柱进行保留、分离，大气压电离源将样品离子化，经串联四极杆复合线性离子阱质谱进行定性和定量检测。茉和酸在0.1-100ng/mL浓度范刚内呈良好线性．实际样品的最低榆出限为0．05ng／mL，最低定量限为0.1ng/ML.通过标准添加的方式，考察高、中、低三个水平的样品凹收率以及相对标准偏差（n=15），得到三个水平的平均回收率分别为80．57％、95．24％、98．48％，相对标准偏差为1．59％、1．26％、1．04％。
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| 关 键 词： | 茉莉酸 植物提取物 HPLC—QTrap |
Identification and Quantitative Determination of Jasmonic Acid in Plant Extracts |
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| JIN Wei,YU Wenjing,ZHENG Li. Identification and Quantitative Determination of Jasmonic Acid in Plant Extracts[J]. Flavour Fragrance Cosmetics, 2013, 0(6): 10-13 |
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| Authors: | JIN Wei YU Wenjing ZHENG Li |
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| Affiliation: | (Suzhou Entry-Exit Inspection and Quarantine Bureau, Suzhou 215104, Jiangsu, China) |
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| Abstract: | A method for determination of jasmonic acid in plant extracts has heen developed. The plant sample was extracted and separated using reversed phase silica columns, then determined by LC-MS/MS. The calibration curves of jasmonie acid showed good linearity in the range of 0.1 to 200 ng/mL, the detection limit of method was 0.05 ng/mL, and the limit of quantifieation was 0. I ng/mL. After adding standard solution to sample, the average recoveries and RSDs of high, medium, low level （n = 15） were investigated. The average recoveries of three levels were 80. 57%, 95.24% and 98.48%, respectively, and the RSDs were 1.59%, 1.26% and 1.04% , respectively. |
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| Keywords: | jasmonic acid plant extracts HPLC-QTrap |
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