| 菌落PCR技术检测化妆品中金黄色葡萄球菌 |
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| 引用本文： | 汪敏,王婷. 菌落PCR技术检测化妆品中金黄色葡萄球菌[J]. 香料香精化妆品, 2013, 0(6): 31-32 |
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| 作者姓名： | 汪敏 王婷 |
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| 作者单位： | [1]国家精细石油化工产品质量监督检验中心／天津市产品质量监督检测技术研究院,天津300384 [2]天津师范大学,天津300387 |
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| 摘 要： | 利用菌落PCR技术,以金黄色葡萄球菌的特有基因femA为靶基因,选择特异引物．进行扩增,鉴定化妆品中金黄色葡萄球菌。琼脂糖凝胶电泳检测PCR产物结果显示,仅在含有金黄色葡萄球菌基因组的样品中得到条带。
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| 关 键 词： | 金黄色葡萄球菌 化妆品 菌落PCR |
Determination of Staphylococcus aureus in Cosmetics by Colony Polymerase Chain Reaction |
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| WANG Min,WANG Ting. Determination of Staphylococcus aureus in Cosmetics by Colony Polymerase Chain Reaction[J]. Flavour Fragrance Cosmetics, 2013, 0(6): 31-32 |
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| Authors: | WANG Min WANG Ting |
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| Affiliation: | 1. National Speciality Petrochemicals Inspection and Testing Center/Tianjin Product Quality Inspection Technology Research Institute, Tianjin 300384, China; 2. Tianjin Normal University, Tianjin 300387, China) |
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| Abstract: | Colony polymerasc chain reaction （PCR） technology was used to detect Staphylococcus aureus in cosmetics. Ac cording to the specific gene of femA of Staphylococcusaureus that had been reported, primers were designed and specific seg ments were cloned. This method was simple, rapid and characterized with acceptable sensitivity and accuracy to meet the re quirements for the analysis of cosmetics. |
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| Keywords: | Staphylococcus aureus cosmelics colony polymerase chain reaction |
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