Electrospun Fibers as a Solid‐State Real‐Time Zinc Ion Sensor with High Sensitivity and Cell Medium Compatibility |
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| Authors: | Jia‐Hao Syu Yi‐Kai Cheng Wun‐Yan Hong Hsing‐Ping Wang Yu‐Chao Lin Hsin‐Fei Meng Hsiao‐Wen Zan Sheng‐Fu Horng Gao‐Fong Chang Chen‐Hsiung Hung Yu‐Cheng Chiu Wen‐Chang Chen May‐Jywan Tsai Henrich Cheng |
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| Affiliation: | 1. Department of Electrical Engineering, National Tsing Hua University, Hsinchu 300, Taiwan, Republic of China;2. Institute of Physics, National Chiao Tung University, Hsinchu 300, Taiwan, Republic of China;3. Department of Photonics and the Institute of Electro‐Optical Engineering, National Chiao Tung University, Hsinchu 300, Taiwan, Republic of China;4. Institute of Chemistry, Academia Sinica, Taipei 115, Taiwan, Republic of China;5. Department of Chemical Engineering, National Taiwan University, Taipei 106, Taiwan, Republic of China;6. Neural Regeneration Laboratory and Center for Neural Regeneration, Department of Neurosurgery, Neurological Institute, Taipei Veterans General Hospital, Taipei 112, Taiwan, Republic of China |
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| Abstract: | meso‐2,6‐Dichlorophenyltripyrrinone (TPN‐Cl2), a probe molecule for zinc II ions, is dispersed in a polymer host. The red fluorescence peak at 620 nm appears when the molecule forms a complex with zinc at its center. TPN‐Cl2 has a high selectivity for zinc II and tolerates many common metal ions present in the human body. The probe molecules are blended with a hydrogel polymer, poly(2‐hydroxyethyl methacrylate) (poly HEMA), with 30 wt% dimethylformamide (DMF). The fiber structure with 1 μm diameter is made by electrospinning in DMF solution of the probe and poly HEMA mixture. The fibrous film detects zinc ions with concentrations as low as 10?6 M in real‐time both in water and in the commonly used cell culture liquid media Dulbecco's modified Eagle medium (DMEM) and fetal bovine serum (FBS), which contain many metal ions and proteins. The time‐resolution is 5 min for 10?6 M and 1 min for 10?5 M. This sensitivity and response speed satisfy the requirements for non‐invasive biomedical studies. |
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| Keywords: | electrospun fibers zinc ion sensors solid‐state materials real‐time detection |
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