The homeobox-containing gene EgHbx3 from Echinococcus granulosus is expressed in the stalk of protoscoleces |
| |
Authors: | C Martínez C Chalar J González R Ehrlich |
| |
Affiliation: | Veterinary Sciences Division, Department of Agriculture for Northern Ireland, Belfast, UK. |
| |
Abstract: | A method is described for the determination of the antibacterial drug trimethoprim in tissues. Minced tissue is homogenised with chloroform-acetone (1 + 1 v/v), filtered, and the filtrate evaporated to an oily residue using a rotary evaporator. The residue is redissolved in methanol-water-acetic acid (50 + 48.7 + 1.3 v/v) and any fats present are partitioned into hexane. The aqueous phase is analysed by liquid chromatography-thermospray mass spectrometry in positive mode with the protonated molecular ion at m/z 291 being monitored. Recoveries ranged between 60% in liver and 79% in muscle. The limit of determination was 25 micrograms kg-1 and the limit of detection was approximately 4 micrograms kg-1. The method is suitable for monitoring tissues taken under national surveillance schemes for veterinary drug residues. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|