| 反相高效液相色谱法测定草莓中的维生素C |
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| 引用本文： | 王卫东.反相高效液相色谱法测定草莓中的维生素C[J].食品科学,1993,14(8):61-63. |
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| 作者姓名： | 王卫东 |
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| 作者单位： | 中国科学院长沙农业现代化研究所 |
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| 摘 要： | 报道了测定草莓中维生素C的反相高效液相色谱法。样品中的维生索C在zorbax ODs（5μm）反相色谱柱上分离、以0．025mol/LKH2PO4-甲醇（70:30）为流动相洗脱、用紫外检测法检测（λ=254 nm）。维生素C在2．5min内得到了良好的分离,检测范围为025mg/L。用于实际样品的分析,效果良好。
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| 关 键 词： | 反相高效液粉色谱 草莓 维生素C |
A Method for the Determination of Vitamin C in Strawberry by Reversed -Phase High Performance Liquid Chromatography |
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| Wang Weidong.A Method for the Determination of Vitamin C in Strawberry by Reversed -Phase High Performance Liquid Chromatography[J].Food Science,1993,14(8):61-63. |
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| Authors: | Wang Weidong |
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| Affiliation: | Wang Weidong |
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| Abstract: | A method for the determination of vitamin c in strawberry by reversed-phase high performance liquid chromatography has been reported in this paper. Vitamin c was separated on a Zorbax ODS (5μm) reversed-phase column using a solution of 0. 025 mol/L KH2PO4: methanol = 70:30 as the mobile phase and detected by ultraviolet spec-trophotometric detector at 254 nm. Good separation of Vitamin c was achieved in 2. 5 min . the range of detection is 0 -25 mg/L. The method was used for the analysis of actual samples and good results were obtained. |
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| Keywords: | Reversed-phase High Performance Liquid Chromatography Strawberry Vitamin C0 |
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