基于植物DNA条形码技术对杏仁露中花生源性成分的鉴别研究

杏仁露含有丰富的植物蛋白,深受广大消费者的喜爱,拥有广阔的消费市场,同时其成分与配料表是否相符也备受消费者关心。本研究基于植物DNA条形码技术与PCR技术,设计、筛选同时能对杏仁、花生、核桃、大豆、芝麻和榛子六个物种进行扩增的通用引物,并将其应用于杏仁露中花生源性成分的检测。试验表明引物ITS2-2和trn H-psb A-1分别对六个物种的扩增成功率和测序成功率均较高;通过计算杏仁、花生的基因组DNA提取率,设计掺假模型在提取的杏仁基因组DNA中掺入花生基因组DNA,引物ITS2-2对于掺入85.80%的花生检测结果为杏仁,trn H-psb A-1对于掺入6.94%的花生检测结果为花生。引物ITS2-2和trn H-psb A-1可作为鉴别杏仁露中花生源性成分的植物DNA条形码组合。本研究为该类食品检测提供了新的思路,可作为相关研究的参考。

Identification of Peanuts in Almond Juice Based on Plant DNA Barcode Technology

Almond juice is rich in plant protein. It is loved by a number of consumers and has a broad consumer market. Meanwhile, whether almond juice was adulterated or not was concerned by consumers. In this study, universal primers for 6 species of almonds, peanuts, almonds, peanuts, walnuts, soybeans, sesame and hazelnuts were designed and screened by using plant DNA barcoding technology and PCR technology to detect the peanuts in almond juice. The results showed that both ITS2-2 and trnH-psbA-1 had higher success rate of amplification and sequencing for six species. A hybrid model was designed with peanut genome DNA mixed in almond genome DNA by calculating the extraction rate of peanut genome DNA and almond genome DNA, and theprimer ITS2-2 in 85.80% peanut was almond and primer trnH-psbA-1 in 6.94% peanut was peanut. ITS2-2 and trnH-psbA-1 could be used as a plant DNA barcode combination to identify peanut in almond juice. This study provides a new idea for the detection of such food, which could be used as a reference for relevant researches.