磁场对水杨酸诱导下红豆杉紫杉醇产量的影响

研究了电磁场对水杨酸(SA)诱导下悬浮培养的南方红豆杉细胞的生长和紫杉醇产量的影响。结果表明在磁场强度为3.5 mT直流稳恒磁场下细胞活力、生物量和胞内蛋白含量均有明显升高;培养液碱化加剧;胞内苯丙氨酸转氨酶(PAL)活力峰值及胞外酚累积均提前。磁场作用后紫杉醇的产量第2天出现大量累积;最高产量出现在第6天,比SA单独处理组提前2天,但产量有所降低。

Effect of magnetic field associated with salicycle acid on taxol production of suspension-cultured Taxus chinensis var. mairei

The effect of direct current magnetic field(DCMF) associated with salicycle acid(SA) on the cell growth and taxol production of suspension-cultured Taxus chinensis var.mairei was studied.The results demonstrate that the cell viability,biomass and intracellular protein are obviously enhanced by the DCMF.The result also shows that the alkalization of medium is aggravated,the activity of the intracellular phenlyalanine ammonia lyase(PAL) and the accumulation of extra-cellular phenolics respond quickly.The taxol commences to accumulate largely in the 2~(nd) day and the accumulation of taxol is 3.4 fold higher and 2.5 fold higher than that of control and cells treated by SA alone,respectively,and the highest accumulation occurs in the 6~(th) day,which is 2 days ahead of that of cells treated by SA alone,however,the accumulation is merely 0.75 fold of that of cells treated by SA alone.