Determination of free fatty acids in marine phytoplankton causing red tides by fluorometric high-performance liquid chromatography |
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| Authors: | Toshiyuki Suzuki Yukihiko Matsuyama |
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| Affiliation: | (1) Nansei National Fisheries Research Institute, 739-04 Saeki-gun, Hiroshima, Japan;(2) Tohoku National Fisheries Research Institute, 3-27-5 Shinhama, Shiogama, 985 Miyagi, Japan |
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| Abstract: | The proportion and content of free fatty acids (FFA) in four species of causative phytoplankton of red tides, cultured in axenic conditions, were obtained by fluorometric high-performance liquid chromatography. Twelve FFA in phytoplankton were identified. Raphidophyte flagellates,Chattonella antiqua andHeterosigma akashiwo, contained highly unsaturated fatty acids and 16:0 acid as the predominant FFA. Major FFA in diatoms,Skeletonema costatum andChaetoceros didymum, were 14:0, 16:0, 16:1, and 20:5 acids. |
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| Keywords: | 9-Anthryldiazomethane axenic Chaetoceros didymum Chattonella antiqua free fatty acid Heterosigma akashiwo HPLC phytoplankton Skeletonema costatum |
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