|
|||||
|
|
| 裙带菜基因工程选择标记的研究 | |
| 引用本文： | 于道展,杨玲玲,姜鹏,秦松,曾呈奎. 裙带菜基因工程选择标记的研究[J]. 高技术通讯, 2003, 13(6): 74-77 |
| 作者姓名： | 于道展 杨玲玲 姜鹏 秦松 曾呈奎 |
| 作者单位： | 1. 中国科学院海洋研究所,青岛,266071;中国科学院研究生院,北京,100039 2. 中国海洋大学生命学院,青岛,266003 3. 中国科学院海洋研究所,青岛,266071 |
| 基金项目： | 中国科学院知识创新工程(KZCX3-SW-215)资助项目。 |
| 摘 要： | 研究了裙带莱幼袍子体对10种选择试剂的敏感性。结果发现裙带莱幼袍子体对氯霉素、潮霉素敏感，对除草剂草丁膦极敏感(2天的LD50为8．57mg/L)。根据统计学的要求得出了这3种选择试剂对裙带莱幼孢子体的半致死剂量和95％可信限。本文的结果能为裙带莱基因工程选择标记的建立提供依据。 |
| 关 键 词： | 裙带菜 幼袍子体 基因工程 选择标记 氯霉素 潮霉素 草丁膦 敏感性 |
Study on Selectable Markers in Genetic Engineering of Undaria pinnatifida |
|
| Yu Daozhan,Yang Lingling,Jiang Peng,Qin Song,Zeng Chengkui. Study on Selectable Markers in Genetic Engineering of Undaria pinnatifida[J]. High Technology Letters, 2003, 13(6): 74-77 | |
| Authors: | Yu Daozhan Yang Lingling Jiang Peng Qin Song Zeng Chengkui |
| Abstract: | The sensitivities of young sporophytes of Undaria pinnatifida to ten selective reagents including chloram-phenicol, hygrcmycin, kanamycin, G418, zeocin, penicillin G, streptomycin, lincomycin, basta and amethopterin were tested. Young sporophytes of U. pinnatifida were found sensitive to chloramphenicol and hygromycin, and very sensitive to basta. LD50 and 95% confidence ranges of these three reagents were calculated separately. The results provide important data in developing selectable markers in genetic engineering of Undaria . |
| Keywords: | Undaria pinnatifida Chloramphenicol Hygromycin Basta Selectable marker |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |