Single step cell lysis/PCR detection of Escherichia coli in an independently controllable silicon microreactor |
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Authors: | Cathy Ke Ann-Marie Kelleher Helen Berney Michelle Sheehan Alan Mathewson |
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Affiliation: | aTyndall National Institute, Lee Maltings, Prospect Row, Cork, Ireland |
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Abstract: | Our studies describe a novel microreactor capable of single step microbial assays involving cell lysis and DNA amplification. The device with an integrated platinum heater and temperature sensor, was fabricated using conventional silicon fabrication technologies and then anodically bonded to a Pyrex lid. Finite element analysis (FEA) and experiments have shown that the temperature uniformity in the microreactor reaction cavity is homogeneous and that the microreactor is capable of fast thermal cycling with heating and cooling rates of 11 and 2.7 °C/s, respectively. The microreactor has novel design features, such as a thermal isolation channel which eliminates thermal cross talk and an inlet/outlet port designed for ease of use. The fabricated microreactor was successfully characterised using a multifunction microbial assay involving cell lysis and PCR in a single step. An assay time of 32 min was achieved. |
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Keywords: | Silicon microreactor Cell lysis PCR Thermal cross talk |
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