黑豆中花色苷水解工艺和定量研究

以黑豆为材料，辅助超声波提取花色苷并进行水解研究，结合单因素和正交试验，确定花色苷水解的最佳工艺组合为超声时间20min、水解时间40min、水解温度90℃、盐酸- 甲醇溶液浓度2mol/L。对水解后的提取物进行HPLC 定量分析，以体积分数10% 甲酸- 水溶液和乙腈为流动相，检测波长520nm，定量线性范围为1～250μg/mL，相关系数大于0.9999。黑豆壳中Dp、Cy、Pn 的含量依次为(31.7340 ± 1.9538)、(895.3267 ±12.9120)、(26.8699 ± 0.8815)mg/100g；黑豆中Dp、Cy、Pn 的含量依次为(1.0338 ± 0.0160)、(76.6534 ± 0.2322)、(2.1283 ± 0.3244)mg/100g。

Optimal Hydrolysis and Quantification of Anthocyanins in Black Soybeans

This paper reports the use of single-factor and orthogonal array design methods to optimize the integrated ultrasonicassisted extraction and hydrolysis of anthocyanins in black soybeans prior to their HPLC quantification. The optimal conditions for extracting and hydrolyzing anthocyanins in black soybeans were determined to be: ultrasonic-assisted extraction for 20 minutes using 2 mol/L hydrochloric acid in a mixture of half methonol and half water (V/V), followed by hydrolysis for 40 minut esat 90 ℃. The mobile phase for HPLC quantification of anthocyanins in black soybeans was a mixture of 10% formic acid aqueous solution and acetonitrile and the detection wavelength was set at 520 nm. The HPLC method exhibited a good linearity over the range from 1 to 250 μg/mL for the determination of delphinidin (Dp), cyaniding (Cy) and peonidin (Pn), with a correlation coefficient of lager than 0.9999. The contents of Dp, Cy and Pn were (31.7340 ± 1.9538), (895.3267 ± 12.9120), (26.8699 ± 0.8815) mg/100 g in black soybean seed coat, and (1.0338 ± 0.0160), (76.6534 ± 0.2322), (2.1283 ± 0.3244) mg/100 g in whole black soybean, respectively.