Enhanced Cellulase Production in Fed-Batch Solid State Fermentation of Trichoderma virideSL-1

A novel fed-batch solid state fermentation process was developed in order to avoid problems associated with high initial nutrient concentration, while retaining advantages from high total effective salt concentration. Cellulase production by Trichoderma viride SL-1 was selected as the experimental model. Ammonium sulphate solution was atomized and fed to the culture by pneumatic transfer. Different feed modes were investigated that resulted in improved enzyme productivity. © 1997 SCI.