固态发酵黑曲霉产单宁酶发酵条件研究

经紫外线诱变黑曲霉菌株筛选出1株高产单宁酶的黑曲霉B0201,利用五倍子为诱导物,固态发酵该菌株得到的单宁酶活力有较大提高。单因素优化试验表明,五倍子用量为8%,初始水分含量为50%,初始pH6.0,温度30℃为优化产酶条件。在优化的条件下,培养96h后产单宁酶酶活力达到了58.2 U/g(干基)。因此,黑曲霉固态发酵产单宁酶具有很大的研究意义及广阔的应用前景。

Conditional Investigation of Aspergillus niger Producing Tannase by Solid-state Fermentation

The Aspergillus niger B0201 producing tannase highly was selected from Aspergillus niger strain being through UV mutagenesis.With gallnut as an inducement in this test,the activity of tannase un- der solid--state fermentation has improved greatly.Optimization experiment being single factor shows that the best condition of tannase production should be in the gallnut amount of 8%,the initial moisture content of 50%,the initial pH 6.0,temperature of 30℃.In the optimization of condition,tannase activity produc- tion reached 58.2U/gds after 96h.Therefore,tannase from Aspergillus niger under solid-state fermentation has a great significance and wide application outlook.