| 基于SSR分子标记的普通菜豆种质遗传多样性分析 |
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| 引用本文： | 夏春阳,杜吉到,韩毅强,孙浩月,李明,吴洪斌,张琦,于晟龙.基于SSR分子标记的普通菜豆种质遗传多样性分析[J].食品与机械,2019(10):232-236. |
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| 作者姓名： | 夏春阳 杜吉到 韩毅强 孙浩月 李明 吴洪斌 张琦 于晟龙 |
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| 作者单位： | 黑龙江八一农垦大学农学院，黑龙江 大庆 163319 |
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| 基金项目： | 黑龙江省农垦总局重点研发项目（编号：HKKY100208）；黑龙江八一农垦大学研究生创新科研项目（编号：YJSCX2019-Y07） |
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| 摘 要： | 建立SSR-PCR反应体系对69份菜豆种质资源进行遗传多样性分析，并利用UPGMA法进行聚类分析及类群划分。17对SSR标记共检测获得等位变异位点55个，变幅2～5个，平均每对检测到标记3.24个；17对引物的多态性位点数17个，多态性百分含量100%。根据聚类分析结果：材料间遗传相似性系数（GS）变异范围0.392～0.963，平均值为0.659；在遗传相似系数0.67水平上将69份菜豆种子材料分为4个类群，说明普通菜豆种质遗传多样性丰富。
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| 关 键 词： | 普通菜豆 SSR标记 遗传多样性 |
| 收稿时间： | 2019/5/10 0:00:00 |
Genetic diversity analysis of common bean germplasms based on SSR molecular markers |
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| XIAChunyang,DUJidao,HANYiqiang,SUNHaoyue,LIMing,WUHongbin,ZHANGQi,YUShenglong.Genetic diversity analysis of common bean germplasms based on SSR molecular markers[J].Food and Machinery,2019(10):232-236. |
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| Authors: | XIAChunyang DUJidao HANYiqiang SUNHaoyue LIMing WUHongbin ZHANGQi YUShenglong |
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| Affiliation: | College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China |
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| Abstract: | The genetic diversity of 69 common bean germplasm resources was analyzed by SSR-PCR reaction system, and cluster analysis was carried out by UPGMA method. Seventeen pairs of SSR markers were detected and 55 allelic mutation loci were obtained, ranging from 2 to 5, with an average of 3.24 markers per pair. Seventeen pairs of primers had 17 polymorphic loci with 100% polymorphism. According to the cluster analysis results, the genetic similarity coefficient (GS) of the material ranged from 0.392 to 0.963, with an average of 0.659. 69 kinds of the beans seeds were divided into four groups at the genetic similarity coefficient of 0.67. This study inclined that the genetic diversity of kidney bean germplasm was rich. |
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| Keywords: | common bean simple sequence repeat genetic diversity |
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