| 在乳腺癌细胞MCF-7中紫杉醇诱导硫氧还蛋白结合蛋白-2的表达 |
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| 引用本文： | 党英男,白洁.在乳腺癌细胞MCF-7中紫杉醇诱导硫氧还蛋白结合蛋白-2的表达[J].昆明理工大学学报(理工版),2009,34(4):73-77. |
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| 作者姓名： | 党英男 白洁 |
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| 作者单位： | 昆明理工大学,生命科学与技术学院,云南,昆明,650224 |
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| 基金项目： | 云南省中青年学术技术带头人后备人才基金资助项目，云南省科技计划项目联合专项 |
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| 摘 要： | 探讨紫杉醇在人乳腺癌细胞株MCF-7中诱导硫氧还蛋白结合蛋白-2表达及与其诱导的相关途径．用不同浓度的紫杉醇刺激MCF-7细胞，MCF-7细胞生长受抑制．通过荧光实时定量PCR检测，发现紫杉醇刺激MCF-7细胞后硫氧还蛋白结合蛋白-2mRNA的表达升高．经荧光素酶活性检测，得出紫杉醇诱导硫氧还蛋白结合蛋白-2基因启动子活性的增高．p38丝裂原激活蛋白激酶（p38MAPK）抑制剂，SB203580抑制了紫杉醇诱导的硫氧还蛋白结合蛋白-2mRNA的表达．因此，紫杉醇抑制MCF-7细胞生长，并通过p38丝裂原激活蛋白激酶途径诱导硫氧还蛋白结合蛋白-2表达．
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| 关 键 词： | 乳腺癌 硫氧还蛋白结合蛋白-2 紫杉醇 癌细胞 |
Expression of Paclitaxel Inducing TRX-Binding Protein-2 in MCF-7 Cells |
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| DANG Ying-nan,BAI Jie.Expression of Paclitaxel Inducing TRX-Binding Protein-2 in MCF-7 Cells[J].Journal of Kunming University of Science and Technology(Natural Science Edition),2009,34(4):73-77. |
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| Authors: | DANG Ying-nan BAI Jie |
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| Affiliation: | ( Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650224, China) |
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| Abstract: | The expression of paclitaxel inducing thioredoxin binding protein - 2 （ TBP - 2 ） and the inducing pathway of TBP- 2 in MCF- 7 cell are firstly discussed in this paper. MCF- 7 cells are treated with paclitaxel at different concentrations and the growth of MCF cells is inhibited. The expression of TBP - 2 induced by paclitaxel is examined by Real -PCR. The activity of TBP -2 reporter is increased by paclitaxel by the Luc assay. The expression of TBP -2 induced by paclitaxel is inhibited by p38 MAPKinase inhibitor, SB203580. Thus, paclitaxel inhibits MCF- 7 cell growth and induces TBP- 2 expression through the p38 MAPkinase pathway. |
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| Keywords: | breast cancer TBP - 2 paclitaxel cancer cells |
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