Bioethanol production from micro-algae, Schizocytrium sp., using hydrothermal treatment and biological conversion |
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Authors: | Jung Kon Kim Byung-Hwan Um Tae Hyun Kim |
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Affiliation: | (1) Department of Chemical and Environmental Engineering, University of Toledo, Toledo, OH 43606, USA; |
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Abstract: | Hydrothermal fractionation for micro-algae, Schizocytrium sp., was investigated to separate sugars, lipids, and proteins. This fractionation process produced protein-rich solid cake
and liquid hydrolysates, which contained oligomeric sugars and lipids. Oligomeric sugars and lipids were easily separated
by liquid-liquid separation. Sugars in the separated hydrolyzate were determined to be mainly D-glucose and L-galactose. Fractionation
conditions were optimized by response surface methodology (RSM). Optimal conditions were found to be 115.5 °C of reaction
temperature, 46.7 min of reaction time, and 25% (w/w) of solid loading. The model predicted that maximum oligomeric sugar
yield (based on untreated micro-algae weight), which can be recovered by hydrothermal fractionation at the optimum conditions,
was 19.4 wt% (based on the total biomass weight). Experimental results were in agreement with the model prediction of 16.6
wt%. Production of bioethanol using micro-algae-induced glucan and E. coli KO11 was tested with SSF (simultaneous saccharification and fermentation), which resulted in 11.8 g-ethanol/l was produced from 25.7 g/l of glucose; i.e. the theoretical maximum ethanol yield based on glucan in hydrolyzate was 89.8%. |
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