山苍子精油抑菌及抗氧化作用的研究

采用超临界CO2萃取法从山苍子中提取精油,用滤纸片法测定山苍子精油对常见细菌、霉菌及酵母的抑菌圈大小,同时研究了pH值及加热处理对山苍子精油抑菌效果的影响,并以过氧化值为指标,测定了山苍子精油对猪油的抗氧化效果。结果表明:山苍子精油对枯草杆菌和白葡萄球菌的抑菌圈处于10～15mm之间,属于中度敏感;对大肠杆菌、黑曲霉、青霉和酵母的抑菌圈达15mm以上,属最敏感;山苍子精油的抑菌效果在pH值5以下强烈,pH值6以上随pH值增大有所减弱;经80、100、121℃加热处理15min对其抑菌效果无明显影响;山苍子精油具有一定的氧化作用,但其抗氧化能力弱于TBHQ。

Study on the Anti-oxidation and Bacteriostasis of Litsea cubeba Extraction Oil

By using supercritical CO2 extraction, Litsea cubeba extraction oil is gotten. Bacteriostasis diameters to bacteria, yeast and molds were assayed with filter paper. And the influences to the bacteriostasis affect by using heating and changing the pH are also investigated. And then, the anti-oxidation effect of Litsea cubeba extraction oil is studied according to the peroxide value. The result showed that: since the bacteriostasis diameters of Litsea cubeba extraction oil on Bacillus subtilis and white Staphylococcus cremorcs are from 10～15mm, and the two bacteria are sensitive to the Litsea cubeba extraction oil. While, bacteriostasis diameters on Escherichia.coli, Aspergillusniger, Penicillium and Saccharmoyces cerevisiae are over 15mm, and the four bacteria are more sensitive to the Litsea cubeba extraction oil. the effect of Litsea cubeba extraction oil to the test organisms are great uner pH5.0, but get weaker with the increasing of pH. Treating the Litsea cubeba extraction oil in 80, 100, 121℃ for 15 mins, the bacteriostasis affect is not changed obviously. Mean while, the Litsea cubeba extraction oil has some kinds of anti-oxidation ability, but is weaker than TBHQ.