Development of Trityl Group Anchored Solubilizing Tags for Peptide and Protein Synthesis |
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Authors: | Dr. Taku Yoshiya Dr. Shugo Tsuda Shun Masuda |
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Affiliation: | Peptide Institute, Inc., 7-2-9 Saito-Asagi, Ibaraki-Shi, Osaka, 567-0085 Japan |
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Abstract: | Recently, solubilizing tag methods (Trt-K and Trt-R method) were developed for the challenging synthesis of peptides/proteins by means of native chemical ligation. In this system, the solubilizing tag can be attached to the Cys side chain by simply mixing the tag-introducing reagent under acidic conditions. The tagged peptides/proteins exhibited high water solubility thanks to the introduction of redundant oligo-Lys/Arg. In the final reaction, the tag can be quickly and cleanly detached by a standard deprotection reaction with trifluoroacetic acid. Herein, the development and application of these methods are described. |
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Keywords: | native chemical ligation peptides proteins synthesis design solubilizing tags |
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