Terminal Deoxynucleotidyl Transferase in the Synthesis and Modification of Nucleic Acids |
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Authors: | Dr. Ivo Sarac Dr. Marcel Hollenstein |
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Affiliation: | Laboratory for Bioorganic Chemistry of Nucleic Acids, Department of Structural Biology and Chemistry, Institut Pasteur, CNRS UMR3523, 28, rue du Docteur Roux, 75724 Paris Cedex 15, France |
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Abstract: | The terminal deoxynucleotidyl transferase (TdT) belongs to the X family of DNA polymerases. This unusual polymerase catalyzes the template-independent addition of random nucleotides on 3′-overhangs during V(D)J recombination. The biological function and intrinsic biochemical properties of the TdT have spurred the development of numerous oligonucleotide-based tools and methods, especially if combined with modified nucleoside triphosphates. Herein, we summarize the different applications stemming from the incorporation of modified nucleotides by the TdT. The structural, mechanistic, and biochemical properties of this polymerase are also discussed. |
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Keywords: | DNA end labeling immobilization nucleotides terminal deoxynucleotidyl transferase |
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