| 电磁脉冲辐射对小鼠睾丸Occludin表达的影响 |
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| 引用本文： | 徐凯,侯武刚,曾丽华,赵洁,李臻,张远强.电磁脉冲辐射对小鼠睾丸Occludin表达的影响[J].辐射研究与辐射工艺学报,2011(5):302-306. |
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| 作者姓名： | 徐凯 侯武刚 曾丽华 赵洁 李臻 张远强 |
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| 作者单位： | 第四军医大学基础部组织学与胚胎学教研室;第四军医大学预防医学系放射医学教研室; |
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| 基金项目： | 国家自然科学基金(No.30771138,No.30871309)资助 |
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| 摘 要： | 探讨电磁脉冲辐射对小鼠睾丸血-睾屏障紧密连接蛋白Occludin表达的变化.采用场强为400 kV/m的辐射场对30只成年雄性Balb/c小鼠进行200次重复全身照射,另30只假照射.于辐照后1、7、14、21和28d分别取小鼠睾丸组织制备石蜡标本或提取组织蛋白和mRNA,使用Real-time PCR、Western...
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| 关 键 词： | Occludin 血-睾屏障 电磁脉冲 小鼠 睾丸 |
Effect of electromagnetic pulses on the expression of occludin in testis of mice |
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| XU Kai HOU Wugang ZENG Lihua ZHAO Jie LI Zhen ZHANG Yuanqiang.Effect of electromagnetic pulses on the expression of occludin in testis of mice[J].Journal of Radiation Research and Radiation Processing,2011(5):302-306. |
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| Authors: | XU Kai HOU Wugang ZENG Lihua ZHAO Jie LI Zhen ZHANG Yuanqiang |
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| Affiliation: | XU Kai1 HOU Wugang1 ZENG Lihua2 ZHAO Jie1 LI Zhen1 ZHANG Yuanqiang1 1 2 |
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| Abstract: | The expression and significance of blood-testis-barriar tight junction protein as well as Occludin in the testis of mice was investigated after whole-body exposure to electromagnetic pulse(EMP).Thirty adult Balb/c mice were irradiated to the whole body with 400kV/m EMP and 200 repeat while other thirty adult Balb/c mice were sham irradiated in the same condition.Specimens were harvested to prepare paraffin-embedded slice or to extract tis-sue protein and mRNA on 1,7,14,21 and 28 days after irradiation.Chang... |
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| Keywords: | Occludin Blood-testis-barrier Electromagnetic pulse(EMP) Mouse Testis |
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