| Penicilliumchrysogenum的低温空气等离子体诱变研究 |
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| 引用本文： | 桂芳,刘会,王辉,王鹏,袁成凌,郑之明.Penicilliumchrysogenum的低温空气等离子体诱变研究[J].辐射研究与辐射工艺学报,2011(5):290-296. |
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| 作者姓名： | 桂芳 刘会 王辉 王鹏 袁成凌 郑之明 |
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| 作者单位： | 中国科学院离子束生物工程学重点实验室; |
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| 基金项目： | 国家高技术研究发展计划(2009AA02Z305)资助 |
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| 摘 要： | 以产黄青霉菌(Penicillium chrysogenum)Pc05为出发菌株,利用物理诱变方法低温空气等离子体技术对其进行诱变处理,结果表明:在处理时间为30 min内时,产黄青霉孢子的存活率随处理时间变化呈现出明显的“马鞍型”曲线；正突变株占所有突变株比例为44.19％,且负突变率很低.经过初筛、复筛获得突变株a...
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| 关 键 词： | 低温空气等离子体 产黄青霉 诱变育种 青霉素 诱变机理 |
Studies of air plasma techniques in mutating Penicillium chrysogenum |
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| GUI Fang LIU Hui WANG Hui WANG Peng YUAN Chengling ZHENG Zhiming.Studies of air plasma techniques in mutating Penicillium chrysogenum[J].Journal of Radiation Research and Radiation Processing,2011(5):290-296. |
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| Authors: | GUI Fang LIU Hui WANG Hui WANG Peng YUAN Chengling ZHENG Zhiming |
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| Affiliation: | GUI Fang LIU Hui WANG Hui WANG Peng YUAN Chengling ZHENG Zhiming(Key Laboratory of Ion Beam Bioengineering,Chinese Academy of Sciences,Hefei 230031,China) |
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| Abstract: | Penicillin producing strain Penicillium chrysogenum Pc05 as the starting strain was mutated by low-temperature air plasma technology.As the result revealed,in 30 minutes,the survival rate of spores followed the saddle-shaped curve.The positive mutants accounted for 44.19% of all mutants while the negative mutation was low.After primary and secondary screening,the mutant aPc051310 was obtained,and eventually its penicillin titer increased 42.1% compared with that of starting strain.Synergetic effect between ... |
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| Keywords: | Low temperature air plasma Penicillium chrysogenum Mutation breeding Penicillin Mutation mechanism |
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