| 99mTc(O)(PNA-dp)辐射切割质粒DNA的性能研究 |
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| 引用本文： | 宋宏涛,魏洪源,杨玉青,王关全,罗顺忠.99mTc(O)(PNA-dp)辐射切割质粒DNA的性能研究[J].辐射研究与辐射工艺学报,2011(4):214-218. |
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| 作者姓名： | 宋宏涛 魏洪源 杨玉青 王关全 罗顺忠 |
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| 作者单位： | 中国工程物理研究院核物理与化学研究所; |
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| 基金项目： | 中国工程物理研究院核物理与化学研究所科技创新基金(2009CX02)中国工程物理研究院科学技术发展基金(2007B02004)资助; 国防基础科研计划(B1520110007); |
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| 摘 要： | 肽核酸(PNA)分子具有特异基因靶向亲和性,用放射性核素标记后,可达到肿瘤靶向和对非靶组织最小损伤的目的,对于基因水平的肿瘤治疗具有十分重要的意义.本工作探索了99mTc标记T基PNA单体所得配合物99mTc (O) (PNA-dp)对常用基因靶向DNA的切割效果.结果表明,配合物在室温密封放置6h,放化纯度大于90％...
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| 关 键 词： | 99mTc 肽核酸 DNA切割 pUC 019 DNA |
Studies on the characteristics of DNA cleavage under the action of ~(99m)Tc(O)(PNA-dp) |
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| SONG Hongtao WEI Hongyuan YANG Yuqing WANG Guanquan LUO Shunzhong.Studies on the characteristics of DNA cleavage under the action of ~(99m)Tc(O)(PNA-dp)[J].Journal of Radiation Research and Radiation Processing,2011(4):214-218. |
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| Authors: | SONG Hongtao WEI Hongyuan YANG Yuqing WANG Guanquan LUO Shunzhong |
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| Affiliation: | SONG Hongtao WEI Hongyuan YANG Yuqing WANG Guanquan LUO Shunzhong(Institute of Nuclear Physics and Chemistry,China Academic of Engineering Physics,Mianyang 621900,China) |
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| Abstract: | Peptide nucleic acid can be used in the field of tumor gene therapy with minimal non-target damage if it binds to gene with high sequence specificity and selectivity when labeled with radionuclides.In this paper,99mTc(O)(PNA-dp) was synthesized,and the characteristics of DNA-cleavage were studied.It shows that the radiochemical purity remains over 90% after 6 h storage at room temperature,and the complex can be injected directly.Besides,the obvious DNA cleavage effect can be found after 1 h,and the mechanis... |
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| Keywords: | 99mTc Peptide nucleic acid(PNA) DNA cleavage pUC 019 DNA |
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