| 高能脉冲电子束诱变筛选丙酮丁醇梭菌及发酵研究 |
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| 引用本文： | 付玉洁,吕江涛,王言,张琴,张敏,张明锋,付海英,孙晓宇.高能脉冲电子束诱变筛选丙酮丁醇梭菌及发酵研究[J].辐射研究与辐射工艺学报,2011(5):297-301. |
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| 作者姓名： | 付玉洁 吕江涛 王言 张琴 张敏 张明锋 付海英 孙晓宇 |
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| 作者单位： | 同济大学生命科学与技术学院;中国科学院上海应用物理研究所; |
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| 基金项目： | 上海市教育委员会科技创新项目(08ZZ21)资助 |
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| 摘 要： | 实验以丙酮丁醇梭菌(Clostridium acetobutylicum)CICC8012为出发菌株,采用高能脉冲电子束(High energy pulsed electron,HEPE)对丙酮丁醇梭菌进行辐照诱变,通过含丁醇的固体培养基对诱变菌株进行胁迫,诱使丙酮丁醇梭菌的丁醇耐受性提高.经过多次诱变和筛选,最终获得...
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| 关 键 词： | 高能脉冲电子束 丙酮丁醇梭菌 丁醇 诱变 筛选 |
Mutagenesis of Clostridium acetobutylicum by HEPE and research of the fermentation |
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| FU Yujie,LU Jiangtao,WANG Yan,ZHANG Qin,ZHANG Min,ZHANG Mingfeng,FU Haiying,SUN Xiaoyu.Mutagenesis of Clostridium acetobutylicum by HEPE and research of the fermentation[J].Journal of Radiation Research and Radiation Processing,2011(5):297-301. |
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| Authors: | FU Yujie LU Jiangtao WANG Yan ZHANG Qin ZHANG Min ZHANG Mingfeng FU Haiying SUN Xiaoyu |
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| Affiliation: | 1(School of Life Sciences and Technology, Tongji University, Shanghai 200092, China) 2(Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China) |
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| Abstract: | This thesis takes Clostridium acetobutylicum CICC 8012 as the original strain and uses HEPE(High energy pulsed electron) to mutate the strain.With progressive cultivation method,a new strain S10 was selected,which has a high production of butanol with 8.32 g/L,increasing 10% compared to the original strain.And then,the experiment of adding CH3CHOONH4,FeSO4 and MnSO4 was carried out to explore if these factors had effect on the fermentation production of butanol.The result showed that these factors could inc... |
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| Keywords: | High energy pulsed electron(HEPE) Clostridium acetobuylicum Butanol Mutation Screening |
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