| 电离辐射和紫杉醇诱导的KB细胞中SPATA5L1和Cyclin B2表达的变化 |
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| 引用本文： | 杨丹,许玉杰,白洁,翁婉雯,丁文秀,洪承皎.电离辐射和紫杉醇诱导的KB细胞中SPATA5L1和Cyclin B2表达的变化[J].辐射研究与辐射工艺学报,2011(4):243-248. |
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| 作者姓名： | 杨丹 许玉杰 白洁 翁婉雯 丁文秀 洪承皎 |
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| 作者单位： | 苏州大学医学部放射医学与公共卫生学院基础核医学教研室;江苏省放射医学与防护重点实验室; |
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| 基金项目： | 国家自然科学基金(30970873); 江苏省高校自然科学基金(04KJB350130); 江苏省放射医学与防护重点实验室开放课题(KJS05030)资助 |
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| 摘 要： | 电离辐射与紫杉醇协同作用，可以诱导体外培养的人口腔上皮癌KB细胞出现G2／M阻滞，形成多核细胞，并最终造成死亡。先前通过基因芯片筛选研究发现，在经过电离辐射和／或紫杉醇作用后，有10条与细胞分裂有关的基因存在差异表达。本研究旨在通过Real—time PCR和Western Blot定量分析其中两条基因SPATA5L1和CyclinB2的表达变化，来揭示G2／M阻滞及多核细胞形成的分子机制。结果表明，在受到6Gy以下不同剂量的电离辐射或电离辐射与40nmol／L紫杉醇协同作用后，SPATA5L1的表达量明显增加，但同时CyclinB2的表达量则下降。推测SPATA5L1上调表达和CyclinB2适度的下调表达，使受处理细胞在胞核分裂后阻止胞浆分裂而无法形成正常子代细胞，导致G2／M阻滞及多核细胞形成。
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| 关 键 词： | 紫杉醇 KB细胞 多核细胞 SPATA5L1 CyclinB2 |
The expression of the SPATA5L1 and Cyclin B2 in KB cell induced by irradiation and paclitaxel |
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| YANG Dan XU Yujie Bai Jie WENG Wanweng DING Wenxiu HONG Chengjiao.The expression of the SPATA5L1 and Cyclin B2 in KB cell induced by irradiation and paclitaxel[J].Journal of Radiation Research and Radiation Processing,2011(4):243-248. |
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| Authors: | YANG Dan XU Yujie Bai Jie WENG Wanweng DING Wenxiu HONG Chengjiao |
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| Affiliation: | YANG Dan1 XU Yujie1 Bai Jie1 WENG Wanweng1 DING Wenxiu1 HONG Chengjiao2 1(Department of Basic Nuclear Medicine,School of Radiation Medicine and Public health,Medical College of Soochow University,Suzhou 215123,China) 2(Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection,China) |
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| Abstract: | The G2/M arrest of human oral epithelial carcinoma KB cells could be induced by the synergistic ef-fect of ionizing radiation with paclitaxel,which results in the formation of multinucleated giant cells and eventually cell death.Earlier biochip screening analysis found that ten genes related to regulate cell division have been differen-tial expression after treated with ionizing radiation and/or paclitaxel.This study was to investigate the potential mo-lecular mechanism by quantitative assessment two gene e... |
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| Keywords: | Paclitaxel KB cell Multinucleated giant cell SPATA5L1 Cyclin B2 |
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