| 内嵌SiO_2纳米颗粒的超大孔复合晶胶的制备(英文) |
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| 引用本文： | 许潘萍,姚雨辰,沈绍传,贠军贤,姚克俭. 内嵌SiO_2纳米颗粒的超大孔复合晶胶的制备(英文)[J]. 中国化学工程学报, 2010, 18(4): 667-671 |
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| 作者姓名： | 许潘萍 姚雨辰 沈绍传 贠军贤 姚克俭 |
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| 作者单位： | 1. State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology, College of Chemical Engineering and Materials Science, Zhejiang University of Technology, Hangzhou 310032, China;2. School of Chemical Engineering, University of New South Wales, Sydney NSW 2052, Australia |
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| 基金项目： | the Natural Science Foundation of Zhejiang Province;the National Natural Science Foundation of China |
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| 摘 要： | Supermacroporous composite cryogels embedded with SiO2 nanoparticles were prepared by radical cryogenic copolymerization of the reactive monomer mixture of acrylamide(AAm) and N,N-methylene-bis-acrylamide(MBAAm) containing SiO2 nanoparticles(mass ratios of nanoparticles to the monomer AAm from 0.01 to 0.08) under the freezing-temperature variation condition in glass columns.The properties of these composite cryogels were measured.The height equivalent to theoretical plate(HETP) of the cryogel beds at different liquid flow rates was determined by residence time distribution(RTD) using tracer pulse-response method.The composite cryogel matrix embedded with the mass fraction of SiO2 nanoparticles of 0.02 presented the best properties and was employed in the following graft polymerization.Chromatographic process of lysozyme in the composite cryogel grafted with 2-acrylamido-2-methyl-1-propanesulfonic acid(AMPSA) was carried out to evaluate the protein breakthrough and elution characteristics.The chromatography can be carried out at relatively high superficial velocity,i.e.,15 cm·min-1,indicating the satisfactory mechanical strength due to the embedded nanoparticles.
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| 关 键 词： | composite cryogel SiO2 nanoparticle protein chromatography |
| 收稿时间： | 2010-01-13 |
| 修稿时间： | 2010-1-13 |
Preparation of supermacroporous composite cryogel embedded with SiO2 nanoparticles |
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| XU Panping,YAO Yuchen,SHEN Shaochuan,YUN Junxian,YAO Kejian. Preparation of supermacroporous composite cryogel embedded with SiO2 nanoparticles[J]. Chinese Journal of Chemical Engineering, 2010, 18(4): 667-671 |
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| Authors: | XU Panping YAO Yuchen SHEN Shaochuan YUN Junxian YAO Kejian |
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| Affiliation: | State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology, College of Chemical Engineering and Materials Science, Zhejiang University of Technology, Hangzhou 310032, China;State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology, College of Chemical Engineering and Materials Science, Zhejiang University of Technology, Hangzhou 310032, China;State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology, College of Chemical Engineering and Materials Science, Zhejiang University of Technology, Hangzhou 310032, China;State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology, College of Chemical Engineering and Materials Science, Zhejiang University of Technology, Hangzhou 310032, China |
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| Abstract: | Supermacroporous composite cryogels embedded with SiO2 nanoparticles were prepared by radical cryogenic copolymerization of the reactive monomer mixture of acrylamide (AAm) and N,N-methylene-bis-acrylamide (MBAAm) containing SiO2 nanoparticles (mass ratios of nanoparticles to the monomer AAm from 0.01 to 0.08) under the freezing-temperature variation condition in glass columns. The properties of these composite cryogels were measured. The height equivalent to theoretical plate (HETP) of the cryogel beds at different liquid flow rates was determined by residence time distribution (RTD) using tracer pulse-response method. The composite cryogel matrix embedded with the mass fraction of SiO2 nanoparticles of 0.02 presented the best properties and was employed in the following graft polymerization. Chromatographic process of lysozyme in the composite cryogel grafted with 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPSA) was carried out to evaluate the protein breakthrough and elution characteristics. The chromatography can be carried out at relatively high superficial velocity, i.e., 15 cm&;#8226;min&;#61485;1, indicating the satis-factory mechanical strength due to the embedded nanoparticles. |
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| Keywords: | composite cryogel SiO2 nanoparticle protein chromatography |
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