Fatty Acid Composition of Non-Starch and Starch Neutral Lipid Extracts of Portuguese Sourdough Bread |
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Authors: | Jo?o M Rocha Paavo J Kalo F Xavier Malcata |
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Affiliation: | 1. CBQF/Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Dr. António Bernardino de Almeida, 4200-072, Porto, Portugal 2. Department of Food and Environmental Sciences, University of Helsinki, P. O. Box 27, Latokartanonkaari 11, 00014, Helsinki, Finland 3. Instituto Superior de Agronomia, Universidade Técnica de Lisboa, Tapada da Ajuda, 1349-017, Lisboa, Portugal 4. Departamento de Fitotecnia, Pólo de Mitra, Escola de Ciências e Tecnologia, Universidade de évora, Apartado 94, 7002-554, évora, Portugal 5. Department of Chemical Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465, Porto, Portugal 6. CEBAL-Centro de Biotecnologia Agrícola e Agro-alimentar do Baixo Alentejo e Litoral, Rua Pedro Soares, Apartado 6158, 7801-908, Beja, Portugal 7. CICECO-Centre for Research in Ceramics and Composite Materials, University of Aveiro, 3810-193, Aveiro, Portugal
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Abstract: | Fatty acids in each neutral lipid (NL) class from non-starch (NSL) and starch lipids (SL) were quantified in maize and rye flours, and sourdough—to ascertain the effect of mixing, and between sourdough and broa (a traditional Portuguese sourdough bread)—to assess the effect of fermentation. Maize and rye flour lipid extracts showed distinct fatty acid profiles. Maize flour exhibited a higher amount of most fatty acid species and of total NL—where triacylglycerols (TAG) and free fatty acids (FA) represented the dominant NL. The fatty acid profiles varied throughout breadmaking (i.e. from the mixture of flours with water, through fermentation until baking): in NSL, the highest concentrations were found in the flour mixture (i.e. 59?% of maize and 41?% of rye flour) or sourdough, whereas in SL they were found in broa; additionally, SL levels increased, and NSL levels decreased during dough preparation and baking. Palmitic (C16:0), oleic (C18:1) and linoleic (C18:2) acids were the major fatty acids in all food items. Cumulative percentages of C16:0, C18:1 and C18:2 in NSL and SL were over 81 and 75?%, respectively, in the flour mixture, 86 and 66?% in sourdough, and 83 and 82?% in broa. In particular, C18:2 accounted for 52?% of the total fatty acids. The content of sterol esters remained essentially constant throughout fermentation and baking; those of TAG decreased from flours to fermentation, while diacylglycerol and monoacylglycerol increased; the NL fractions were essentially unaffected by baking, yet their FA contents increased. |
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