| 豆瓣中产磷脂酶菌株的筛选及系统发育分析 |
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| 引用本文： | 董丹,蒋丽,关统伟,车振明,曾雷.豆瓣中产磷脂酶菌株的筛选及系统发育分析[J].中国酿造,2015,34(3):44-47. |
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| 作者姓名： | 董丹 蒋丽 关统伟 车振明 曾雷 |
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| 作者单位： | 西华大学生物工程学院，西华大学微生物研究所，食品生物技术四川省高校重点实验室，四川成都610039 |
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| 基金项目： | 教育部春晖计划项目(No.13205639);食品生物技术四川省高校重点实验室(No.Szjj2013-045);四川省教育厅基金项目资助(No.13205688) |
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| 摘 要： | 以一级郫县豆瓣为原料筛选磷脂酶高产菌株，通过磷脂平板初筛和摇瓶复筛法，以磷脂酶水解圈作为筛选模型，并对产酶菌株进行了酶活力的检测及细菌16S rRNA系统发育分析。结果表明，从一级豆瓣中筛选出10株产磷脂酶菌株，其中产酶活力最高的为编号LZ8，酶活力为68.32 U/mL，经鉴定该菌株为Bacillus sonorensis，属于芽孢杆菌属，其与最高同源性菌株相似性为100%。
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| 关 键 词： | 磷脂酶 系统发育分析 酶活力 芽孢杆菌属 |
Screening and phylogenetic analysis of phospholipase producing strains in fermented soybean paste |
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| DONG Dan;JIANG Li;GUAN Tongwei;CHE Zhenming;ZENG Lei.Screening and phylogenetic analysis of phospholipase producing strains in fermented soybean paste[J].China Brewing,2015,34(3):44-47. |
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| Authors: | DONG Dan;JIANG Li;GUAN Tongwei;CHE Zhenming;ZENG Lei |
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| Affiliation: | Key Laboratory of Food Biotechnology in Colleges and Universities in Sichuan Province, Institute of Microbiology,
College of Bioengineering, Xihuan University, Chengdu 610039, China |
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| Abstract: | In this experiment, the first class Pixian soybean paste was used to screen phospholipase-producing strain. The high phospholipase-producing strain was isolated by phospholipase screening plates and shakes flask affiliation, and identified through 16S rRNA sequence analysis. The results showed that ten phospholipase-producing strains were screened out from first class Pixian soybean paste, where the strain named LZ8 had the highest phospholipase-producing activity of 68.32 U/ml. The strain was identified as Bacillus sonorensis, of 100% homology with the highest homology strains. |
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| Keywords: | phospholipase phylogenetic analysis enzyme activity Bacillus |
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