六味地黄汤抑制大鼠生精细胞凋亡及其促进精子发生

用醋酸棉酚建造大鼠生精障碍模型,用六味地黄汤灌胃治疗,以市售六味地黄丸和甲基睾酮分别为对照和阳性对照,TUNEL法检测实验各组大鼠睾丸生精细胞的凋亡情况,计数实验各组大鼠精子数量,考察实验各组大鼠精子的质量.结果表明,六味地黄汤各治疗组、阳性对照组大鼠的精子数量、精子爬高均与模型组有显著性差异(P<0.01),各治疗组大鼠的精子质量(活力)也显著优于模型组;各实验组大鼠睾丸凋亡的生精细胞明显少于模型组.由此显示,六味地黄汤复方可显著抑制大鼠睾丸生精细胞的凋亡,对生精障碍大鼠有显著的促进生精作用,并可显著提高大鼠精子的质量.

Effect of Liuwe Dihuang Decoction on Promoting Spermatogenesis and Inhibiting Spermatogonium Apoptosis of Rat

The model of spermatogenesis obstructive rat was built with gossypol acetate. Then Liuwei Dihuang decoction and methyltestosterone were administered to the Wistar rats. The numbers of sperm were counted, the quality of sperm was observed, and the spermatogonium apoptosis was tested by TUNEL. Result showed that the sperm quantity of the rats in LW treated groups was significantly different to that of the model group(P<0.01),and higher than that of the positive group.The sperm quality of the rats in LW treated groups was better than that of the model group.The number of spermatogonium apoptosis of LW treated groups was smaller than that of the model group. It could be concluded that LW could promote the quantity and quality of the sperm and inhibit the spermatogonium apoptosis of the rats in model group.