Effect of feeding different diets on the exocrine pancreatic secretion of nitrogen,amino acids and enzymes in growing pigs

Five barrows were fitted with permanent pancreatic re-entrant cannulas to determine the influence of diet on exocrine pancreatic secretions in the weight range from 40 to 65 kg. The pigs were fed four diets. Diets 1 and 2 were based on corn starch and contained 18.6 and 16.9 % crude protein (dry matter basis) from soybean meal and canola meal, respectively. Diets 3 and 4 contained 93.9 % wheat and barley, respectively. In the same order, the crude protein contents (dry matter basis) of these diets were 14.4 and 11.2%, respectively. The experiment was carried out according to 4 × 4 Latin square design. Each experimental period comprised 12 days. Following an adaptation period of 8 days, pancreatic juice was collected from 08:00 on day 9 to 08:00 h on day 10 and from 08:00 on day 11 to 08:00 h on day 12. The pigs were fed twice daily, at 08:00 and 20:00 h, 800 g each meal. Despite large differences, up to 23%, between the overall mean values of the parameters that were measured there was no effect (P > 0.05) of diet on the volume of secretion of pancreatic juice, the pH and the secretion of total and TCA-precipitable nitrogen. Due to the high level of within-study variation there was also no effect (P > 0.05) of diet on the secretion of amino acids and the total activity of trypsin, chymotrypsin, α-amylase and lipase in pancreatic juice.