| 离子注入选育L(+)-乳酸耐酸菌及去中和剂发酵研究 |
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| 引用本文： | 李文,王陶,余增亮.离子注入选育L(+)-乳酸耐酸菌及去中和剂发酵研究[J].食品与发酵工业,2008,34(12). |
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| 作者姓名： | 李文 王陶 余增亮 |
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| 作者单位： | 1. 徐州工程学院食品工程学院,江苏,徐州,221008
2. 中国科学院离子束生物工程学重点实验室,安徽,合肥,230031 |
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| 基金项目： | 国家高技术研究发展计划(863计划)，徐州工程学院校级重点课题 |
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| 摘 要： | 为了获得可用于原位分离藕联发酵的耐乳酸菌株,以米根霉RE3303为出发菌株,在投糖浓度为50g/L,氮源为3.0g/L尿素的条件下,进行离子注入选育,得到L(+)-乳酸耐酸菌株RK4002,不添加中和剂,经30h的摇瓶发酵,L(+)-乳酸产量达32.32g/L,产率达1.08g/(L.h),为出发菌株的1.7倍,且可在24g/L的耐乳酸平板上生长,具有一定的应用潜力。
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| 关 键 词： | L-乳酸 米根霉 耐酸菌 离子注入 去中和剂发酵 |
L(+)-lactic Acid by Ion Beam Implantation and the Fermentation Without Neutralization |
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| Li Wen,Wang Tao,Yu Zengliang.L(+)-lactic Acid by Ion Beam Implantation and the Fermentation Without Neutralization[J].Food and Fermentation Industries,2008,34(12). |
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| Authors: | Li Wen Wang Tao Yu Zengliang |
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| Abstract: | In order to get a strain which can bear high lactic acid for ISPR(in situ product removal)under the fermentation condition of glucose 50g/L,Urea 3.0g/L,the original strain Rhizopus oryzae RE3303 was mutated by ion beam implantation.A mutant RK4002 bearing higher lactic acid was screened,the concentration of L(+)-lactic acid reached 32.32 g/L after 30 h shake-f1ask cultivation without any neutralization.The productivity was 1.08g/(L·h),which was a 146% increase compared with the original strain.RK4002 could also grow on PDA plate containing 24g/L lactic acid,which showed potential for industrial use. |
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| Keywords: | L-lactic acid Rhizopus oryzae bearing high L(+)-lactic acid ions implantation fermentation without neutralization |
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