| 邻苯二甲醛同步荧光衍生化法测定微量指血中还原型谷胱甘肽 |
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| 引用本文： | 张小勇,黄成泽,朱爱花,崔胜云.邻苯二甲醛同步荧光衍生化法测定微量指血中还原型谷胱甘肽[J].延边大学理工学报,2012(2):146-149. |
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| 作者姓名： | 张小勇 黄成泽 朱爱花 崔胜云 |
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| 作者单位： | 延边大学长白山生物资源与功能分子教育部重点实验室,吉林延吉133002 |
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| 基金项目： | 国家自然科学基金资助项目（21165021）; 吉林省教育厅项目（吉教科合字2010D273） |
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| 摘 要： | 采用微升（μL）级微量指血为检测样品,在未对样品去蛋白的条件下,用邻苯二甲醛（OPA）荧光衍生化法测定了微量静脉血中还原型谷胱甘肽（GSH）的含量.利用标准曲线法和标准加入法进行3次测定的均值分别为4.415μmol/L和5.417 5μmol/L,加标回收率为95.28%～97.18%,检测限为3.6×10-8 mol/L,可基本满足微量成分分析所要求的准确度.本研究为灵敏、简便和快速测定微量血样中GSH的含量提供了实验和方法学的依据.
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| 关 键 词： | 谷胱甘肽 邻苯二甲醛 荧光光度法 衍生化 |
Glutathione determination by finger blood sampling using o-phthaladehyde as fluorescent derivatizing agent |
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| ZHANG Xiao-yong,HUANG Cheng-ze,ZHU Ai-hua,CUI Sheng-yun.Glutathione determination by finger blood sampling using o-phthaladehyde as fluorescent derivatizing agent[J].Journal of Yanbian University (Natural Science),2012(2):146-149. |
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| Authors: | ZHANG Xiao-yong HUANG Cheng-ze ZHU Ai-hua CUI Sheng-yun |
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| Affiliation: | ( Key Laboratory of Natural Resources of the Changbai Mountain ＆ Functional Molecules (Yanbian University), Ministry of Education, Yanji 133002, China ) |
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| Abstract: | Reduced glutathione is sensitively determinined by fluorescent spectrometry using o-phthalaldehyde （OPA） as fluorescent derivatizing agent and different calibration methods by micro sampling of finger blood without protein elimination. The average amounts of GSH determined by calibration and standard addition methods are 4. 415 μmol/L and 5. 417 5μmol/L respectively, recoveries are in the range of 95.28% 97.18%, detection limits is 3.6 × 10-8 mol/L. The methods is suitable for simple, rapid and sensitive determination of GSH in micro blood sample. |
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| Keywords: | glutathione o-phthaladehyde fluorescent spectrometry derivatization |
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