| 积雪草多糖的脱脂工艺研究 |
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| 引用本文： | 韦媛媛,刘阳,龚帅,刘鹏. 积雪草多糖的脱脂工艺研究[J]. 广西工学院学报, 2014, 0(2): 90-94 |
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| 作者姓名： | 韦媛媛 刘阳 龚帅 刘鹏 |
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| 作者单位： | 广西科技大学生物与化学工程学院,广西柳州545006 |
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| 基金项目： | 广西教育厅科研项目（201106LX384）;地方高校国家级大学生创新创业训练计划项目（201310594014）资助 |
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| 摘 要： | 为优选并确立积雪草的最佳脱脂工艺,考察积雪草不同脱脂溶剂对多糖得率和脱脂率的影响,通过单因素试验和正交试验对脱脂工艺进行优化.最佳脱脂工艺为：料液比（g/mL）1∶10,乙醚-乙醇溶液（V乙醚∶V乙醇=2∶3）在35℃下脱脂6h.在此脱脂方案下,积雪草多糖平均得率为2.69％（n=5）,RSD为2.23％,脱脂率为15.87％（n=5）.该脱脂方案稳定,可使多糖得率更高.
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| 关 键 词： | 积雪草 多糖 脱脂工艺 |
Study on degreasing process of polysaccharides from entella asiatica |
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| WEI Yuan-yuan,LIU yang,GONG Suai,LIU Peng. Study on degreasing process of polysaccharides from entella asiatica[J]. Journal of Guangxi University of Technology, 2014, 0(2): 90-94 |
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| Authors: | WEI Yuan-yuan LIU yang GONG Suai LIU Peng |
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| Affiliation: | (College of Biological and Chemical Engineering, Guangxi University of Science and Technology, Liuzhou 545006, China) |
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| Abstract: | Objective： To study the optimization of degreasing conditions on polysaccharides from Centella asiatica. Method： The effect of different degreasing agents on polysaccharide yield of Centella asiatica ？and ratio？of degreasing was studied. The optimal conditions of degreasing conditions of Centella asiatica were studied through single-factor and orthogonal experiments. Result： The optimal degreasing conditions are as follows： the ratio of material to solvent was 1：10, the degreasing agent was ether and alcohol （v： v=2：3）, and the degreasing time was 6 hour at 35℃. Under these conditions, the yield of polysaccharide in Centella asiatica was 2.69% （n=5）, RSD was 2.23%, the ratio of degreasing was 15.87% （n=5）. Conclusion： The degreasing conditions of polysaccharides in Centella asiatica was stable and efficient. |
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| Keywords: | centella asiatica polysaccharide degreasing process |
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