Facile preparation and cytocompatibility of poly(lactic acid)/poly(3‐hydroxybutyrate‐co‐4‐hydroxybutyrate) hybrid fibrous scaffolds |
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| Authors: | Xinhui Wang Lin Sang Zhiyong Wei Lijie Zhai Hong Wang Kedong Song Min Qi |
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| Affiliation: | 1. School of Materials Science and Engineering, Dalian University of Technology, , Dalian, 116024 China;2. Department of Polymer Science and Materials, Dalian University of Technology, , Dalian, 116024 China;3. First Affiliated Hospital of Dalian Medical University, , Dalian, 116011 China;4. Dalian R&D Center for Stem Cell and Tissue Engineering, State Key Laboratory of Fine Chemicals, Dalian University of Technology, , Dalian, 116024 China |
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| Abstract: | A fibrous scaffold is required to provide three‐dimensional (3D) cell growth microenvironments and appropriate synergistic cell guidance cues. In this study, porous scaffolds with different mass ratio of poly(lactic acid) to poly(3‐hydroxybutyrate‐co‐4‐hydroxybutyrate) (P(3HB‐co‐4HB)) for tissue engineering were prepared by a modified particle leaching method. The effect of the addition of P(3HB‐co‐4HB) on microstructural morphology, compression property, swelling behavior, and enzymatic degradation of hybrid scaffolds was systematically investigated. The results indicated that this method was simple but efficient to prepare highly interconnected biomimetic 3D hybrid scaffolds (PP50/50 and PP33/67) with fibrous pore walls. The cytocompatibility of hybrid scaffolds was evaluated by in vitro culture of mesenchymal stem cells. The cell‐cultured hybrid scaffolds presented a complete 3D porous structure, thus allowing cell proliferation on the surface and infiltration into the inner part of scaffolds. The obtained hybrid scaffolds with pore size ranging from 200 to 450 µm, over 90% porosity, adjustable biodegradability, and water‐uptake capability will be promising for cartilage tissue engineering applications. POLYM. ENG. SCI., 54:2902–2910, 2014. © 2014 Society of Plastics Engineers |
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