一氯均三嗪型染料与蚕丝纤维键合的HPLC研究

采用高效液相色谱法定量研究一氯均三嗪型活性染料与酪氨酸模拟蚕丝纤维的结合情况,从而探讨活性染料与蚕丝纤维的键合机制。通过测定不同温度、pH值条件下染料的水解和酪氨酸的电离色谱峰面积,对活性染料与酪氨酸结合情况进行研究。结果表明:活性染料的水解量与色谱峰面积有较好的线性关系;活性染料与蚕丝纤维的模拟物酪氨酸的反应程度与酪氨酸的电离率及未水解的活性染料两者均有关,在pH值为9.5、温度85℃附近,活性红K-2BP与酪氨酸的结合率最大。

HPLC study of the bonding of MCT reactive dye with silk fiber

HPLC was adopted to perform a quantitative research on the bonding of MCT reactive dye with silk fiber which was simulated by tyrosine,and their bonding mechanism was investigated.The bonding of reactive dye with tyrosine at different temperatures and different pH values was examined through testing the hydrolysate of the reactive dye and ionization chromatogram peak area of tyrosine. It shows that there is a linear relation between the hydrolyzing ratio of reactive dye and HPLC peak area.The reaction degree of reactive dyes with tyrosine is related with both ionization degree of tyrosine and non-hydrolyzed dyes.When pH value is 9.5,temperature is(85 ℃,)the strongest bonding between Reactive Red K-2BP and tyrosine is achieved.