| 固定化重组大肠杆菌产1,3-丙二醇发酵条件的研究 |
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| 引用本文： | 张晓梅,诸葛健.固定化重组大肠杆菌产1,3-丙二醇发酵条件的研究[J].食品与发酵工业,2007,33(3):23-26. |
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| 作者姓名： | 张晓梅 诸葛健 |
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| 作者单位： | 江南大学生物工程学院,江南大学工业生物技术教育部重点实验室,江苏无锡,214036 |
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| 摘 要： | 利用海藻酸钙将重组大肠杆菌JM109(pHsh-dhaB-dhaG-dhaF-yqhD)固定化,并对影响重组菌固定化细胞发酵的营养因子进行研究。实验结果表明,该重组菌固定化细胞发酵的适宜培养基组成为:甘油80 g/L、酵母膏5.0 g/L、VB120.05 g/L以及KH2PO47.5 g/L;在此培养条件下,1,3-丙二醇产量、转化率及生产能力分别可达61.5 g/L、76.8%和2.57 g(L.h),与游离细胞相比,重组菌固定化细胞对底物甘油和产物1,3-丙二醇的耐受力明显提高,且1,3-丙二醇产量明显提高。
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| 关 键 词： | 1 3-丙二醇 固定化细胞 重组大肠杆菌 |
| 修稿时间： | 11 15 2006 12:00AM |
Study on the 1,3-propanediol Fermentation Parameters by Immobilized Cells of the Recombinant Escherichia coli |
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| Zhang Xiaomei,Zhuge Jian.Study on the 1,3-propanediol Fermentation Parameters by Immobilized Cells of the Recombinant Escherichia coli[J].Food and Fermentation Industries,2007,33(3):23-26. |
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| Authors: | Zhang Xiaomei Zhuge Jian |
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| Affiliation: | The school Biotechnology Southern Yangtz University, The Key Laboratory of Industrial Biotechnology Ministry of Education, Wuxi 214036, China |
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| Abstract: | The immobilization of recombinant strain E.coli JM109(pHsh-dhaB-dhaG-dhaF-yqhD) with calcium algite was firstly performed in this study.Some nutrient ingredient affecting fermentation results of this recombinant strain were also studied.The results demonstrated that the optimized medium comprised(g/L) glycerol 80,yeast extract 5.0,KH2PO4 7.5 and Vitamin B12 0.05.The yield,conversation rate and productivity under the optimal parameters could reach 61.5 g/L,76.8 % and 2.57 g/(L·h),respectively.The experiment result show that the initial glycerol concentration and 1,3-propanediol yield were significantly increased by immobilization. |
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| Keywords: | 1 3-propanediol immobilized cells recombinant Escherichia coli |
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