重组HBcAg与HBsAg混合抗原的免疫效果

目的观察重组HBcAg与HBsAg混合抗原免疫BALB/c小鼠后体液免疫与细胞免疫的效果。方法配制含Al(OH)3佐剂与不含佐剂的混合抗原,分别免疫BALB/c小鼠,采用ELISA法测定抗体滴度和细胞因子水平;采用流式细胞仪分析T细胞亚群。结果HBcAg与HBsAg混合抗原免疫小鼠产生的IFN-γ量明显高于二者单独免疫,CD3+和CD4+水平显著增高;含Al(OH)3佐剂的混合抗原CD8+水平明显高于阴性对照组;混合抗原免疫小鼠血清产生的抗-HBs抗体与2倍剂量的HBsAg单独免疫相比,差异无显著意义。含Al(OH)3佐剂与不含佐剂的疫苗免疫效果差异无显著意义。结论重组HBcAg与HBsAg混合抗原表现出良好的体液免疫与细胞免疫效果,为治疗性乙肝疫苗的开发提供了依据。

Immune Effect of Recombinant HBcAg and HBsAg Mixed Antigen

Objective To observe the humoral and cellular immune effects of recombinant HBcAg and HBsAg mixed antigen in mice.Methods Immunize BALB/c mice with the prepared adjuvant-free and aluminium hydroxide adjuvant-containing recombinant HBcAg and HBsAg mixed antigens respectively.Determine the serum antibody and cytokine levels of the mice by ELISA,and analyze the T cell subgroups by flow cytometry.Results The IFN-γ,CD3+ and CD4+ levels of mice immunized with mixed antigen were significantly higher than those with HBcAg or HBsAg only.The CD8+ level of mice immunized with aluminium hydroxide adjuvant-containing mixed antigen was significantly higher than that with physiological saline as control.The serum anti-HBs level of mice immunized with mixed antigen showed no significant difference from that with HBsAg at double dosage.No significant difference was observed between the immune effects of adjuvant-free and aluminium hydroxide adjuvant-containing antigens.Conclusion Recombinant HBcAg and HBsAg mixed antigen showed good humoral and cellular immune effects,which provided a basis for the development of therapeutic hepatitis B vaccine.