Chromatographic characterization of internal polar lipids from wool |
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Authors: | L Coderch A de la Maza C Soriano P Erra J L Parra |
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Affiliation: | (1) C.I.D. (C.S.I.C.), Jordi Girona 18-26, 08034 Barcelona, Spain |
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Abstract: | Wool internal polar lipids were isolated and separated into different fractions based on polarity. Qualitative and quantitative
analyses of the different fractions were performed by thin-layer chromatography and thin-layer chromatography coupled to flame-ionization
detection, respectively. Cholesterol esters, free fatty acids, sterols, ceramides, glycosylceramides, and cholesterol sulfate
were the main components, with ceramides being in the highest proportion. The fatty acid composition of ceramides and glycosylceramides
was determined by gas chromatography/mass spectrometry. As for other keratinized tissues, long-chain fatty acids predominated
in comparison to either free fatty acids or phospholipid-linked fatty acids; in both cases, stearic and lignoceric acids were
the most abundant fatty acids, and a low amount of 18-methyleicosanoic acid was found. This work opens new avenues in the
study of lipid rearrangement in more complex and realistic vesicle structures than conventional liposomes. |
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Keywords: | Ceramides GC/MS polar lipids TLC/FID wool |
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