| 斑茅F1品系崖城96-66与CP84-1198杂交后代的鉴别 |
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| 引用本文： | 杨业后,符成,张垂明,邓海华,廖兆周. 斑茅F1品系崖城96-66与CP84-1198杂交后代的鉴别[J]. 甘蔗糖业, 2002, 0(4): 11-14 |
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| 作者姓名： | 杨业后 符成 张垂明 邓海华 廖兆周 |
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| 作者单位： | 广州甘蔗糖业研究所,广州,510316 |
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| 摘 要： | 对斑茅F1品系崖城96-66×CP84-1198杂交后代进行过氧化物酶和酯酶同工酶分析,并与父母本酶带作比较。结果表明,受检的20个株系中,除YCE01-62不具斑茅的特征酶带外,其它19个株系都具有斑茅的酶带,可初步认为是斑茅的第二代杂种。对其中7个株系进行了DNA分子标记分析,结果显示除YCE01-62为杂株外,其它株系均确认为真杂种。在本研究中,同工酶方法的结果与DNA分子标记方法的结果相似。
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| 关 键 词： | 斑茅 杂交后代 鉴别 甘蔗 属间发交 同工酶 DNA分子标记 |
Identification of Intergeneric Hybrids from a Cross of Erianthus F1 Clone YC96-66 with CP84-1198 |
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| Yang Yehou,Fu Cheng,Zhang Chuiming,Deng Haihua,Liao Zhaozhou. Identification of Intergeneric Hybrids from a Cross of Erianthus F1 Clone YC96-66 with CP84-1198[J]. Sugarcane and Canesugar, 2002, 0(4): 11-14 |
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| Authors: | Yang Yehou Fu Cheng Zhang Chuiming Deng Haihua Liao Zhaozhou |
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| Abstract: | Intergeneric hybrids (BC1) from a cross of Erianthus F1 clone YC96-66 with CP84-1198 were identified with isozyme and DNA marker analysis. Results from peroxidase isozyme and esterase isozyme analysis showed that 19 of the 20 progeny clones being tested displayed Erianthus-specific bands as their female parent YC96-66 did, and were considered as F2 hybrids of Erianthus arundinareus. The rest one clone, YCE01-62 was suspected to be a rogue seedling since no Eurianthu bands were deleted. DNA markers were employed to confirm 7 of these clones, and the results indicated that all of them were genuine hybrids except YCE01-62. Isosyme and DNA marker analysis gave similar conclusions in identifying progeny from Fl and the commercial variety in this study. |
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| Keywords: | Sugarcane Erianthus arundinaceus Intergeneric Hybridization Isozymes DNA markers |
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