溶氧探测补料技术发酵生产类人胶原蛋白的研究

在重组大肠杆菌发酵生产类人胶原蛋白(HLC)过程中,快速确定不同反应器中的补料速率,抑制乙酸产生,高水平表达HLC.采用溶氧探测补料技术,在不同规模反应器中,分批-补料培养重组大肠杆菌生产HLC,根据脉冲补料方式时溶氧信号的响应特征来辩识是否产生乙酸并确定补料速率.在实验室规模获得的最终细胞密度和HLC质量浓度分别为6...

Fed-batch production of human-like collagen with recombinant Escherichia coli using feed-up DO-transient control

To control the acetate production and achieve a high-level expression of the target protein in the different-scale bioreactors during fed-batch production of human-like collagen(HLC) with recombinant Escherichia coli.A probing feeding strategy using feed-up DO-transient control was employed in two different-scale bioreactors and the dissolved oxygen response signal was used to detect the acetate production in pulsed fed-batch cultivation of E.coli.The final dry cell weight(DCW) of 69.1 g/L and HLC mass conc...