|
|||||
|
|
| 异戊酰螺旋霉素高速逆流色谱中的溶剂体系 | |
| 引用本文： | 顾芨芨,陈葵,朱家文,郝玉有,王维娜,张晓勇,杨生武. 异戊酰螺旋霉素高速逆流色谱中的溶剂体系[J]. 化学工程, 2011, 39(10): 77-81 |
| 作者姓名： | 顾芨芨 陈葵 朱家文 郝玉有 王维娜 张晓勇 杨生武 |
| 作者单位： | 1. 华东理工大学化学工程研究所,上海,200237 2. 上海同田生物技术有限公司,上海,201203 3. 同联集团有限公司,辽宁沈阳,110044 |
| 基金项目： | 上海市科委生物医药重点科技攻关项目(08431900900) |
| 摘 要： | 可利霉素是转基因工程菌发酵所得的一种多组分抗生素药物,分离纯化其作为主要组分的各异戊酰螺旋霉素对药物分析及药理研究具有重要意义.高速逆流色谱作为一种基于液液分配原理的新型分离技术,可用于异戊酰螺旋霉素的分离制备.文中通过测定不同溶剂体系中目标物的分配系数,筛选适合分离制备异戊酰螺旋霉素的溶剂体系.以正己烷-乙酸乙酯-乙... |
| 关 键 词： | 异戊酰螺旋霉素 高速逆流色谱 分配系数 分离因子 |
Solvent system for separation of isovalerylspiramycins in high speed counter-current chromatography |
|
| GU Ji-ji,CHEN Kui,ZHU Jia-wen,HAO Yu-you,WANG Wei-na,ZHANG Xiao-yong,YANG Sheng-wu. Solvent system for separation of isovalerylspiramycins in high speed counter-current chromatography[J]. Chemical Engineering, 2011, 39(10): 77-81 | |
| Authors: | GU Ji-ji CHEN Kui ZHU Jia-wen HAO Yu-you WANG Wei-na ZHANG Xiao-yong YANG Sheng-wu |
| Affiliation: | GU Ji-ji1,CHEN Kui1,ZHU Jia-wen1,HAO Yu-you1,WANG Wei-na2,ZHANG Xiao-yong2,YANG Sheng-wu3(1.Research institute of Chemical Engineering,East China University of Science and Technology,Shanghai,200237,China,2.TAUTO Biotech Co.Ltd.,Shanghai 201203,3.Tonglian Group Co.,Ltd.,Shenyang 110044,Liaoning Province,China) |
| Abstract: | Bitespiramycin is a new antibiotic with multi-components fermented by transgenosis engineering bacteria.To separately prepare isovaleryspiramycins functioned as major component of bitespiramycin is significant in medicine analysis and quality control.High speed counter-current chromatography(HSCCC),as a new purification technology based on liquid-liquid distribution,provides a method for the separation of isovalerylspiramycins.The partition coefficients of isovalerylspiramycins in a series of solvent system... |
| Keywords: | isovalerylspiramycin high speed counter-current chromatography partition coefficient separation factor |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |