| SPR生物传感器在急性白血病髓系抗原CD33检测中的应用 |
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| 引用本文： | 谢永红,王耀玲,程小丽,张玮,王红理,方湘怡.SPR生物传感器在急性白血病髓系抗原CD33检测中的应用[J].传感技术学报,2011,24(1):1-4. |
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| 作者姓名： | 谢永红 王耀玲 程小丽 张玮 王红理 方湘怡 |
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| 作者单位： | 谢永红,张玮,王红理,方湘怡,XIE Yonghong,ZHANG Wei,WANG Hongli,FANG Xiangyi(西安交通大学理学院,实验物理中心,西安,710049);王耀玲,WANG Yaoling(西安市第二人民医院妇产科,西安,710049);程小丽,CHENG Xiaoli(西安交通大学第一附属医院,血液学研究中心,西安,710049) |
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| 基金项目： | 西安市科技计划项目资助 |
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| 摘 要： | 采用自组装膜技术,在传感芯片表面修饰抗CD33单克隆抗体,利用自行构建的SPR生物传感器检测人骨髓血液中急性白血病髓系抗原CD33的表达.实验结果表明SPR生物传感器对AML患者骨髓血液的响应值远远大于对健康人骨髓血液的响应值.与传统的流式细胞术(FCM)方法相比,SPR方法具有能快速给出定量分析结果,操作简单等优点....
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| 关 键 词： | SPR生物传感器 CD33 自组装膜 抗CD33单克隆抗体 急性髓系白血病 |
Application of SPR biosensors in the detection of acute myeloid leukemia associated-antigen CD33 |
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| XIE Yonghong,WANG Yaoling,CHENG Xiaoli,ZHANG Wei,WANG Hongli,FANG Xiangyi.Application of SPR biosensors in the detection of acute myeloid leukemia associated-antigen CD33[J].Journal of Transduction Technology,2011,24(1):1-4. |
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| Authors: | XIE Yonghong WANG Yaoling CHENG Xiaoli ZHANG Wei WANG Hongli FANG Xiangyi |
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| Affiliation: | XIE Yonghong1,WANG Yaoling2,CHENG Xiaoli3,ZHANG Wei1,WANG Hongli1,FANG Xiangyi11.Experimental Physics Center,School of Science,Xi'an Jiaotong University,Xi'an 710049,China,2.The Second hospital of Xian City,3.Hematology Research Center of the First Hospital Affiliated to Xi'an Jiaotong University |
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| Abstract: | A spectral SPR biosensor was used for the detection of acute myeloid leukemia-associated antigen CD33 cells in human bone marrow.The specific monoclonal antibody CD33-Ab was immobilized on the surface of a SPR sensor chip using the technology of self-assembly.The experimental results show that the bone marrow of AML patients reveals much higher expression level of CD33 than those of healthy people.The results of the responses to different type bone marrow show that the selectivity of the SPR biosensors is g... |
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| Keywords: | CD33 |
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