膜分离技术提取海藻糖的工艺

采用超滤、纳滤操作对酵母抽提物进行处理，结果表明：操作压力、操作时间及料液体积流量对超滤有很大影响，所选MWCO为5000的膜件可去除96％以上的大分子蛋白质，起到纳滤预处理作用．采用MWCO为300的纳滤膜对超滤液进行浓缩纯化，海藻糖总提取率高达85．6％，大大高于传统方法．操作条件如进料压力、浓缩倍数及操作方式对纳滤过程均有很大影响。

Trehalose Extraction by the Technology of Membrane Separation

The extraction of yeast extract by ultrafiltration and nanofiltration were studied with an attempt to develop a possible separation and purification process. The results indicated that 96% of proteins was blocked by the membrane of MWCO 5000. The operation pressure, time, and flow velocity had great influences on the ultrafiltration operation process. On the other side, fluid was concentrated and purified through nanofiltration membranes of MWCO 300, operation conditions such as pressure, concentration time and different operation process were studied in the paper. Under the determined conditions, the total trehalose extraction rate was 85.6% and much higher than that of the traditional methods.