不同剂量电离辐射对小鼠树突状细胞(DC)表型及功能的影响

采用流式细胞术和酶联免疫吸附试验(ELISA)检测0.075 Gy及2.0 Gy X射线照射6、12、24、48、72 h后DC/TLC共培养体系中小鼠树突状细胞(DC)表面CD80、CD40分子表达及其分泌细胞因子IL-12、IL-27的变化,探讨电离辐射对DC在免疫反应中的作用。结果显示共刺激分子CD80和CD40及细胞因子IL-12在0.075 Gy照射后与对照组相比表达量均升高,而2.0 Gy照射后其表达量则降低;但IL-27在高、低剂量照射后其表达量均增加。上述结果表明,电离辐射在体外可以诱导DC表型变化及细胞因子分泌的变化,该结果将为辐射免疫效应提供新的实验依据。

Effect of Different Doses of Ionizing Radiation on Phenotype and Function of Dendritic Cells

To study the effect of ionizing radiation on Dendritic Cells(DC),Flow Cytometry(FCM) and Enzyme-linked Immune-sorbent Assay(ELISA) were used to detect the expression of costimulatory molecules CD40 and CD80 on DC as well as their secretion of IL-12 and IL-27 in DC/TLC co-culture system after having been exposed to 0.075 Gy and 2.0 Gy X-rays for 6 h,12 h,24 h,48 h and 72 h respectively.The results demonstrated that 0.075 Gy X-rays can induce CD80 and CD40 on DC and stimulate IL-12 secretion,while they were suppressed by 2.0 Gy.However,IL-27 secretion by DC was stimulated after both low and high doses of radiation.These results suggested that ionizing radiation can cause changes of the phenotype and secretion of cytokines,and this will provide new scientific basis for the study of immune effect following ionizing irradiation.