Immune evasion by tickborne and host-adapted Borrelia burgdorferi |
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Authors: | AM de Silva E Fikrig E Hodzic FS Kantor SR Telford SW Barthold |
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Affiliation: | Department of Chemistry, Biochemistry, Concordia University, 1455 de Maisonneuve Blvd. West, Montréal, Québec H3G 1M8, Canada. joycep@vax2.concordia.ca |
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Abstract: | The Candida glabrata ADE2 gene encoding aminoimidazole ribonucleotide (AIR) carboxylase (EC 4.1.1.21) was isolated by complementation of the ade2-1 mutation in Saccharomyces cerevisiae. The predicted amino acid (aa) sequence is 75% identical to that of S. cerevisiae. Integrative transformation was used to produce a C. glabrata strain bearing a deletion of ADE2 coding sequences. A high-copy-number shuttle vector bearing the ADE2 gene was constructed and contains a fragment of S. cerevisiae mitochondrial (mt) DNA that confers the ability to replicate autonomously in C. glabrata. |
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