Protein-Mediated Transformations of Superparamagnetic Nanoparticles Evidenced by Single-Particle Inductively Coupled Plasma Tandem Mass Spectrometry: A Disaggregation Phenomenon |
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| Authors: | Jacek Sikorski,Magdalena Matczuk,Agnieszka Kamiń ska,Joanna Kruszewska,Maciej Trzaskowski,Andrei R. Timerbaev,Maciej Jarosz |
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| Affiliation: | 1.Chair of Analytical Chemistry, Faculty of Chemistry, Warsaw University of Technology, Noakowskiego St. 3, 00-664 Warsaw, Poland; (J.S.); (A.K.); (J.K.); (M.J.);2.Centre for Advanced Materials and Technologies CEZAMAT PW, Warsaw University of Technology, Poleczki St. 19, 02-822 Warsaw, Poland;3.Vernadsky Institute of Geochemistry and Analytical Chemistry, Kosygin St. 19, 119991 Moscow, Russia; |
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| Abstract: | Progress toward translating superparamagnetic iron oxide nanoparticles (SPIONs) with specific diagnostic and therapeutic properties for clinical applications depends on developing and implementing appropriate methodologies that would allow in-depth characterizations of their behavior in a real biological environment. Herein, we report a versatile approach for studying interactions between SPIONs and proteins using single-particle inductively coupled plasma tandem mass spectrometry. By monitoring the changes in the size distribution upon exposure to human serum, the formation of stable protein corona is revealed, accompanied by particle disaggregation. |
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| Keywords: | superparamagnetic iron oxide nanoparticles human serum single-particle inductively coupled plasma tandem mass spectrometry |
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