| 前列腺素E1冻干隐形脂质体包封率的测定 |
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| 引用本文： | 时念秋,;张大同,;王东凯,;关世侠,;陈宇洲,;陈修毅.前列腺素E1冻干隐形脂质体包封率的测定[J].山东食品科技,2009(1):42-44. |
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| 作者姓名： | 时念秋 ;张大同 ;王东凯 ;关世侠 ;陈宇洲 ;陈修毅 |
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| 作者单位： | [1]山东轻工业学院化学工程学院,山东济南250353; [2]沈阳药科大学药学院,辽宁沈阳110016; [3]广州瑞济生物技术有限公司,广东广州510730; [4]天津中医药大学中药学院,天津300193; [5]山东医药工业研究所,山东济南250100 |
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| 摘 要： | 目的评价前列腺素E1（PGE1）冻干隐形脂质体的质量，测定PGE1冻干隐形脂质体的包封率。方法用超滤法分离脂质体和游离药物；采用HypersilC18柱（250mm×4．6mm，5μm），流动相：pH4．9的磷酸二氢钾溶液-乙腈（3：2），流速1．0mL·min^-1，检测波长210nm，测定药物含量，计算包封率。结果超滤法能很好的分离脂质体与游离药物，PGE1在1～50mg·L^-1内线性关系良好（r=0．9993），平均包封率为93．02％。结论超滤-HPLC法测定PGE1冻干隐形脂质体包封率方法简便、准确。
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| 关 键 词： | 超滤法 高效液相色谱法 前列腺素E1 隐形脂质体 包封率 |
Determination of Entrapment Efficiency of Freeze-dried PGE1 Stealth Liposomes |
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| Affiliation: | SHI Nian-qiu, ZHANG Da-tong, WANG Dong-kai, GUAN Shi-xia, CHEN Yu-zhou, CHEN Xiu-yi(1. College of Chemical Engineering, Shandong Institute of Light Industry, Jinan 250353, China; 2. College of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China; 3. Guangzhou Regex Biotech Co., Ltd, Guangzhou 510730, China; 4. College of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China; 5. Shandong Institute of Pharmaceutical Industry, Jinan 250100, China) |
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| Abstract: | Objective To evaluate the quality of freeze-dried prostaglandin E1（PGE1） stealth liposomes and determine the entrapment efficiency. Methods Liposomes and dissociative drug were separated by ultrafiltration. The content ofPGE1 was determined by Hypersil C18 column （250 mm× 4.6 mm, 5 μm） with the mixture of KH2PO4（PH 4.9）-acetonitrile（3 ： 2） as mobile phase at the flow rate of 1.0 mL · min^-1. The detection wavelength was 210 nm. The entrapment efficiency was calculated. Results It was good to separate liposomes and dissociative drug by ultrafiltration. There was a good linear relationship （r=0.999 3） when the concentration of PGE1 was 1 -50 mg · L^-1. The average entrapment efficiency was 93.02 %. Conclusion The ultrafiltration-HPLC method is convenient and accurate for the determination of entrapment efficiency of freeze-dried PGE1 stealth liposomes. |
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| Keywords: | ultrafiltration HPLC prostaglandin El stealth liposomes entrapment efficiency |
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