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爱普生1640扫描仪和QS2000软件对醋酸纤维膜血清蛋白电泳图谱分析
引用本文:王梅华,朱良臣,曹颖平,张敬喜,郑华燊.爱普生1640扫描仪和QS2000软件对醋酸纤维膜血清蛋白电泳图谱分析[J].福建分析测试,2005,14(1):2137-2139.
作者姓名:王梅华  朱良臣  曹颖平  张敬喜  郑华燊
作者单位:福建医科大学附属协和医院检验科,福建,福州,350001;福建医科大学检验系98级学生,福建,福州,350001
摘    要:评估爱普生1640扫描仪和HELENA公司的QS2000软件是否适用于手工血清蛋白醋酸纤维膜电泳图谱的分析。对各18名肝硬化、肾病综合征患者及18名正常人的血清,以醋酸纤维膜为介质进行电泳,染色,漂洗,石蜡油透明;同时用HELENA SPIFE全自动快速电泳仪进行血清蛋白电泳作对照,分别得到透明的醋酸纤维膜与透明的琼脂糖胶片,再用HELENA公司的QS2000软件对电泳图谱进行分析比较。两种方法电泳得出的结果无显著性差异。爱普生1640扫描仪和QS2000软件可应用于血清蛋白醋酸纤维膜电泳图谱的分析,该方法科学、简便、成本低,具很好的推广价值。

关 键 词:全自动血清蛋白电泳  醋酸纤维膜  扫描  QS2000软件分析

Analysis of serum protein electrophoresis in the acetate fibre membrane by EPSON 1640 scan and the QS2000 software
WANG Meihua,Zhu Liangchen,Cao Yingping,Zhang Jingxi,Zheng Huashen.Analysis of serum protein electrophoresis in the acetate fibre membrane by EPSON 1640 scan and the QS2000 software[J].Fujian Anal Ysis & Testing,2005,14(1):2137-2139.
Authors:WANG Meihua  Zhu Liangchen  Cao Yingping  Zhang Jingxi  Zheng Huashen
Abstract:To evaluate whether EPSON 1640 scan and the QS2000 software of HELENA company can be applicable for analyzing electrophoresis of the transparent acetate fibre membrane by manual work.Three series of serum from each 18 patients with cirrhosis of liver,nephritic syndrome and healthy adults were electrophoresised, coloured,decoloured and transparented by Paraffin oil in the medium of acetate membrane and also were electrophoresised by the HELENA SPIFE Automatic Analysing Instrument.The results were analyzed by EPSON 1640 scan and QS2000 software of HELENA.The results analyzed by this two methods are not significant difference.EPSON 1640 scan and QS2000 software of HELENA may use to analyze electrophoresis of the transparent acetate membrane.
Keywords:Automatic serum albumen electrophoresis  Acetate membrane  Scaning  QS2000 software Analysis
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