响应面法优化超声协同复合酶分步提取恰玛古多糖工艺

以恰玛古多糖得率为指标,在超声提取及复合酶酶解单因素实验基础上,采用响应面法探究超声协同复合酶分步提取恰玛古多糖的最佳工艺条件。结果表明,超声协同复合酶分步提取恰玛古多糖的最佳提取工艺为:液料比33:1 mL/g,超声温度62℃,超声功率250 W,超声提取43 min后加入2.5%的复合酶(纤维素酶:木瓜蛋白酶:果胶酶=1:1:1,质量比),酶解pH5.4,酶解温度50℃,酶解时间52 min,在此条件下,恰玛古多糖得率为12.62%±0.18%。超声协同复合酶提取恰玛古多糖的得率较高,且工艺简便易行,适用于恰玛古多糖的提取。

Optimization of Ultrasonic Synergistic Complex Enzymes for Stepwise Extraction of Brassica rapa L. Polysaccharide by Response Surface Methodology

Based on the single factor experiments of ultrasonic extraction and complex enzyme hydrolysis,the response surface analysis was used to optimize the conditions for the stepwise extraction of Brassica rapa L.polysaccharides by ultrasonic synergistic complex enzyme using extraction rate of Brassica rapa L.polysaccharides as the index. Results showed that,the optimal extraction process of ultrasonically synergistic compound enzyme for stepwise extraction of Brassica rapa L. polysaccharides was as follows:Liquid to material ratio 33:1 mL/g,ultrasonic temperature 62℃,ultrasonic power 250 W,ultrasonic extraction for 43 min,and addition of 2.5% of the complex enzyme(cellulase:papain:pectinase=1:1:1,w/w),enzymatic hydrolysis pH5.4,enzymatic hydrolysis temperature 50℃,enzymatic hydrolysis time 52 min. Under these conditions,the yield of Brassica rapa L. polysaccharide was 12.62%±0.18%. Ultrasonic synergistic compound enzyme stepwise extraction of Brassica rapa L. polysaccharides had a high yield,and the process was simple and easy to apply,which would be suitable for the extraction of Brassica rapa L.polysaccharides.