3种不同溶剂中根皮苷与牛血红蛋白的相互作用及抗氧化性

在dH₂O、10% DMSO和10% EtOH溶剂体系中,利用紫外光谱、荧光光谱、同步荧光光谱和红外光谱法及对DPPH和ABTS自由基清除法,研究牛血红蛋白与根皮苷的相互作用及对根皮苷抗氧化性的影响。结果表明:在3种不同溶剂体系中,随根皮苷浓度的不断增加,其对牛血红蛋白的荧光猝灭均逐渐增强,猝灭机理均为静态猝灭和非辐射能量转移,相互作用力以范德华力和氢键为主,根皮苷与牛血红蛋白的结合位点更接近Trp残基。在dH₂O体系中,酰胺Ⅰ带和Ⅱ带发生轻微红移。除10% EtOH-DPPH体系外,根皮苷-牛血红蛋白均增加了根皮苷对DPPH和ABTS自由基的清除率。该研究表明,溶剂极性可不同程度影响根皮苷与牛血红蛋白的相互作用和抗氧化性。

Interaction and Antioxidant Activities of Phlorizin and Bovine Hemoglobin in Three Different Solvents

The interaction of bovine hemoglobin(BHb)and phlorizin(PHL)and its effects on antioxidant activities of PHL were investigated using ultraviolet spectra,fluorescence spectra,synchronous fluorescence spectra,infrared spectra methods and DPPH and ABTS free radical scavenging assays in deionized water(dH₂O),10%dimethyl sulfoxide(DMSO)and 10% ethanol(EtOH),respectively. The results showed that PHL had a great ability to quench BHb's fluorescence with the increase of PHL concentration,quenching mechanism was an on-radiative energy transfer in static modes,interaction force was driven mainly by Vander Waals forces and hydrogen bond,and PHL was much closer to Trp residue in three different solvents. The slight red shifts for amide band Ⅰ and amide band Ⅱ were observed in dH₂O. The scavenging effects of PHL-BHb on DPPH and ABTS free radical all increased compared with PHL except for 10% EtOH-DPPH. This study showed that the interaction of BHb and PHL and its activities could be influenced by solvents polarity in different degrees.